Akademik Veri Yönetim Sistemi
ICASEM IV. Uluslararası Uygulamalı Bilimler, Mühendislik ve Matematik Kongresi, Tekirdağ, Türkiye, 20 - 23 Ekim 2022, ss.56
The
ACTN3 gene encoding the protein α actinin-3 is possibly associated with human
physical performance. The gene has been examined in a wide spectrum, from
exercise adaptation, exercise recovery, sporting injury risk to athletic
performance, including speed, strength, power and endurance. The goal of this
study was to examine the frequency distribution of ACTN3 R577X polymorphism in
elite Turkish marathon runners and football players.
71
professional football players (age = 24,31 ± 4,60; weight = 75,87 ± 5,85;
height = 178,81 ± 5,79; sporting age= 13,82 ± 4,34) and 34 elite marathon
runners (age = 25,18 ± 4,08; weight = 62,45+5,98; height = 172,89 ± 5,37;
sporting age = 12,94 ± 4,24) were included in the study.
Human
genomic DNA was isolated from blood stains by phenol–chloroform extraction.
Genotyping of the ACTN3 R577X polymorphism was determined for 71 Turkish
football players, 34 Turkish marathon runners, and 56 control subjects by PCR
technics. PCR fragments were digested with DdeI restriction enzyme. Individuals with the RR genotype had bands of
205 and 85 bp, and those with the XX genotype had bands of 108, 97 and 85 bp.
The genotype distribution of ACTN3 R577X was found to be 43.7% RR, 40.8% RX,
15.5% XX and allele frequency 64.1% R allele, and 35.9% X allele for Turkish football
players.
The
genotype distributions and allele frequency in elite Turkish marathon runners
was 32.4% RR, 47.1% RX, 20.6% XX, and 55.9 R allele, 44.1% X allele,
respectively. However, no statistical significance was found between Turkish
football players and Turkish marathon runners for the ACTN3 genotype
distribution and allele frequency (p>0.05). Our findings confirmed that the
ACTN3 gene is similar for different sport disciplines, such as marathon and
football, though they are very distinct over mechanical and metabolic
requirements.